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KMID : 0881720150300020173
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Journal of Food Hygiene and Safety
2015 Volume.30 No. 2 p.173 ~ p.177
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Development of Analytical Methods of Hyperoside from Rosa canina L.
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Oh Jae-Myoung
Lee Hwa-Jung
Bahn Kyeong-Nyeo
Seo Il-Won
Lee Young-Joo
Lee Jin-Hee
Park Ji-Min
Kang Tae-Seok
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Abstract
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Rosa canina L. is health functional food materials that can help to temporarily relieve symptoms of arthritis. This study has been conducted to develop and validate analytical methods for hyperoside of Rosa canina L.. Methods based on HPLC with ultraviolet detection (UVD) were established through instrumental analytical conditions, and the examination of data, such as domestic and foreign reliable methods and journals. HPLC UVD analysis using Capcell Pak C18 MG II column at 353 nm was determined on test through the column, mobile phase. The validation has been performed on the method to determine linearity, accuracy, limits of quantification (LOQ) and repeatability for hyperoside. The method showed high linearity in the calibration curve at a coefficient of correlation (R2 ) of 0.999, and the LOQ was 0.393 μg/mL. Relative standard deviation (RSD) values of data from repeatability precision was between 0.6 and 2.6%. Recovery rate test at hyperoside scored between 98 and 99%. These results indicate that the established HPLC method is very useful for the determination of hyperoside in Rosa canina L. to develop a health functional material.
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KEYWORD
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Health functional food, Rosa Canina L., hyperoside, HPLC, method validation
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